rat anti flag  (Novus Biologicals)

Journal: eLife

Article Title: A split-GAL4 driver line resource for Drosophila neuron types

doi: 10.7554/eLife.98405

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-FLAG rat monoclonal DYKDDDDK Epitope Tag Antibody , Novus Biologicals: NBP1-06712 , RRID: AB_1625981 , 1:200.

Techniques: Software

Journal: bioRxiv

Article Title: The C-terminus of the TlpD cytoplasmic chemoreceptor promotes polar localization and function

doi: 10.1101/2024.11.08.622596

Figure Lengend Snippet: (A) Western blot of E. coli DH10B strains expressing TlpD from a plasmid were analyzed from whole-cell lysates with an anti-FLAG antibody which recognizes the C-terminal tag. (B) Whole-cell lysates of H. pylori G27 Δ tlpD strains complemented with tlpD constructs were similarly analyzed. The TlpD variant expressed is indicated at the top, with TlpD indicating full length with the 3XFLAG. Marker sizes are given in kilodaltons on the right. The expected size of TlpD is 51 kDa, ΔN4 is 45 kDa, ΔN5 is 39 kDa, ΔC3 is 38 kDa, and ΔC4 is 45 kDa. Western blots are representative of 3 biological replicates.

Article Snippet: PVDF membranes were incubated with a 1:20,000 dilution of rat anti-FLAG monoclonal antibody (Novus Biological, NBP1-06712) or a 1:4,000 to 1:10,000 dilution of rabbit anti-TlpA22 antibody [ ] diluted in 5% BSA at 4°C overnight.

Techniques: Western Blot, Expressing, Plasmid Preparation, Construct, Variant Assay, Marker

Journal: bioRxiv

Article Title: The C-terminus of the TlpD cytoplasmic chemoreceptor promotes polar localization and function

doi: 10.1101/2024.11.08.622596

Figure Lengend Snippet: Immunolocalization of H. pylori whole cells producing full-length or truncated TlpDΔC4 protein. H. pylori was detected with chicken anti- H. pylori followed by goat anti-chicken conjugated to AlexaFluor 594 (red). TlpD proteins indicated above each image were detected with preabsorbed rabbit anti-TlpA22 (ΔABCD, TlpD) or rat anti-FLAG (TlpD, Δ4) followed by goat anti-rabbit or goat anti-rat conjugated to AlexaFluor 488 (green). Scale bar = 4μM. Images are representative of 3 biological replicates, with 20-50 bacterial cells analyzed for each sample.

Article Snippet: PVDF membranes were incubated with a 1:20,000 dilution of rat anti-FLAG monoclonal antibody (Novus Biological, NBP1-06712) or a 1:4,000 to 1:10,000 dilution of rabbit anti-TlpA22 antibody [ ] diluted in 5% BSA at 4°C overnight.

Techniques: